To study the progress of syphilis and other venereal diseases, we would vivisect the women at various intervals after they were infected. It was important to understand the effects of the disease on living organs, and vivisection also provided valuable surgical practice. The vivisection was sometimes done with chloroform, sometimes not. We usually vivisected the subjects for the anthrax and cholera experiments without use of anesthesia since anesthesia might have affected the results, and it was felt that the same would be true with the women with syphilis.